Inhibitory Effects of Taxol and Mitomycin C on Proliferation of Cultured Rabbit Subconjunctival Fibroblasts.

Baek, Yong Woo; Moon, Jung Il; Ahn, Myung Douk

Original Article

Journal of the Korean Ophthalmological Society 2003;44(3):727-731.
Published online March 1, 2003.

Inhibitory Effects of Taxol and Mitomycin C on Proliferation of Cultured Rabbit Subconjunctival Fibroblasts.

Yong Woo Baek, Jung Il Moon, Myung Douk Ahn

Department of Ophthalmology, St. Mary's Hospital, The Catholic University of Korea College of Medicine, Korea. jimoon@cmc.cuk.ac.kr

Taxol과 Mitomycin C가 배양된 가토의 결막하 섬유모세포 증식 억제에 미치는 영향

백용우 ( Yong Woo Baek ) , 문정일 ( Jung Il Moon ) , 안명덕 ( Myung Douk Ahn )

Abstract

PURPOSE
Scar tissue formation by proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated inhibitory effects of Mitomycin C (MMC) and Taxol on proliferation of cultured rabbit subconjunctival fibroblasts. METHODS: Tenon's fibroblasts of rabbits were cultured and exposed to various concentrations of each drug for 6 days. Cell viability was measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. RESULTS: Both MMC and Taxol inhibited proliferation of fibroblasts significantly in a dose-dependent manner, with ID50s of 0.27 mg/l and 0.08 mg/l, respectively. CONCLUSIONS: We have confirmed that Taxol is as potent inhibitor of fibroblast proliferation as Mitomycin C. This result may possibly support the use of Taxol in trabeculectomy to improve the success rate of trabeculectomy.

Key Words: Fibroblast;Mitomycin C;Taxol