Journal of the Korean Ophthalmological Society 2003;44(5):1205-1211.
Published online May 1, 2003.
The Effects of Tranilast(R) on the Cellular Proliferation, Collagen Synthesis, and Secretion of TGF-beta1 in Bovine Retinal Pigment Epithelial Cells.
Jong Soo Lee, Young Gun Shin, Jae Ho Jung, Boo Sup Oum
1Department of Ophthalmology, Busan National University College of Medicine, Korea. jongsool@pusan.ac.kr
2Shin sea-kyae eye Clinic, Korea.
Tranilast(R)가 망막색소상피세포의 증식, 교원질 합성, TGF-β1 분비에 미치는 영향
이종수 ( Jong Soo Lee ) , 신영건 ( Young Gun Shin ) , 정재호 ( Jae Ho Jung ) , 엄부섭 ( Boo Sup Oum )
Abstract
PURPOSE
To evaluate the effects of Tranilast(R) on the cellular proliferation, collagen synthesis, and secretion of transforming growth factor-beta1 on retinal pigment epithelial cells that influence the development of proliferative vitreoretinopathy in vitro. METHODS: After bovine RPE cells were isolated, they were exposed to Tranilast(R) 0.05, 0.1, 0.2, 0.4, 0.8, 1.0, 1.6 mg/ml and only DMEM which was used as control. MTT was used as a measurement of metabolic activity, and collagen synthesis and TGF-beta1 secretion were assayed by collagen kit (Sigma, USA) and TGF-beta1 kit (Gibco, USA). Western blot assay was used to confirm TGF-beta1, which was expressed by treated Tranilast(R) in bovine RPE cells. RESULTS: The higher the concentration of Tranilast(R), the more the inhibition of the cellular proliferation. LD50 concentration was about 1.0 mg/ml, and there was more significant difference the concentration of over Tranilast(R) 0.4 mg/ml than control (P<0.05). The higher the concentration of Tranilast(R), the more the inhibition of collagen synthesis and TGF-beta1 secretion from RPE cells, especially over Tranilast(R) 0.05 mg/ml (P<0.05). There was no response of TGF-beta1 expression in the concentration of Tranilast(R) 0.2 mg/ml compared to untreated bovine retinal epithelial cells. CONCLUSIONS: Tranilast(R) has a tendency of inhibitory effect in the cellular proliferation, collagen synthesis, and TGF-beta1 secretion. Tranilast(R) may prevent excessive proliferation of RPE and scar formation concerned with collagen synthesis and TGF-beta1 secretion in the proliferative vitreoretinal diseases.
Key Words: Collagen synthesis;Retinal pigment epithelial cell;TGF-beta1;Tranilast(R)


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