| The Metabolic and Morphological Effects of Transforming Growth Factor (TGF)-beta1 in Bovine Retinal Pigment Epithelial Cells. |
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Jong Soo Lee, Ji Eun Lee, Hyun Jun Park, Boo Sup Oum |
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Department of Ophthalmology, Busan National University College of Medicine, Korea. jongsool@pusan.ac.kr |
| TGF-β1에 의한 망막색소상피세포의 대사능력과 형태학적 변형에 관한 효과 |
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이종수 ( Lee Jong Su ) , 이지은 ( Lee Ji Eun ) , 박현준 ( Park Hyeon Jun ) , 엄부섭 ( Eom Bu Seob ) |
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| Abstract |
PURPOSE
To evaluate the effects of transforming growth factor (TGF)-beta1 on the cellular metabolic activity and morphological changes on retinal pigment epithelial cells that influence the development of proliferative vitreoretinopathy. METHODS: After bovine RPE cells were isolated, they were exposed to TGF-beta1 0.1 ng/ml, 0.4 ng/ml, 0.8 ng/ml, 1.6 ng/ml and DMEM that was used only as control. After 24 hours, MTT based colorimetric assay was performed to assess the inhibition of cellular proliferation, and the cellular morphology was evaluated by inverse phase-contrast light microscope and electron microscope. RESULTS: The higher the concentration of TGF-beta1 with fetal bovine serum, the more the inhibition of the cellular proliferation in bovine RPE cells, especially at the concentration of TGF-beta1 1.6 ng/ml (P<0.05). There was no relationship between the concentration of TGF-beta1 and the cellular metabolic activity in fetal bovine serum free (P>0.05). In TGF-beta1 10 ng/ml, the bovine retinal pigment epithelial cell showed a bundle of cytoplasmic microtendon, disposed parallel to the long axis of the cell and pinocytotic like vesicle. CONCLUSIONS: TGF-beta1 has a tendency of inhibitory effect in the cellular proliferation of bovine RPE cells. We confirmed that there were some characteristics of myofibroblast transformation from RPE treated with TGF-beta1 under TEM. |
| Key Words:
Cellular proliferation;Myofibroblast;Retinal pigment epithelial cell;TGF-beta1 |
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