Journal of the Korean Ophthalmological Society 2003;44(9):2122-2127.
Published online September 1, 2003.
The Effect of Mitomycin C on Cultured Human Osteoblasts.
Jung Ho Lee, Wha Sun Chung, Jun Hyuk Son
Department of Ophthalmology, Yeungnam University College of Medicine, Daegu, Korea. chungwha@med.yu.ac.kr
배양된 인체의 골모세포에 대한 Mitomycin C의 효과
이정호 ( Lee Jeong Ho ) , 정화선 ( Jeong Hwa Seon ) , 손준혁 ( Son Jun Hyeog )
Abstract
PURPOSE
To evaluate the effect of mitomycin-C (MMC) on osteotomy site as an adjunctive therapy for dacryocystorhinostomy, the effect of MMC on cultured human osteoblasts was tested. METHODS: Cultured osteoblasts which was obtained from the human iliac crest, were treated with four different concentrations of MMC (0 mg/ml, 0.2 mg/ml, 0.02 mg/ml, 0.002 mg/ml) and cultured for 24hours. To observe the effect of exposed time dependency, cells were treated with MMC during 5, 30minutes, and 24hours and washed and changed with fresh osteogenic media, and then cultured for 24 hours. The effect of fibroblast growth factor (FGF) and transforming growth factor-beta(TGF-beta) on the MMC-treated cells was evaluated. Cell viability was measured using trypan blue staining method and MTT assay. RESULTS: As compared with control group, the lowest growth rate of osteoblasts was 6.8% in 0.2 mg/ml MMC-treated cells. There were no significant differences in the growth rate between 5 minutes and 30 minutes MMC treatment groups, but in case of 24 hours treatment group with MMC (0.2 mg/ml) the growth rate was suppressed to 77.5% of control group with statistical significance. Both growth factors had promotive effect on the growth of in 0.02 mg/ml MMC-treated osteoblasts, but not in 0.2 mg/ml MMC-treated cells. CONCLUSIONS: Osteoblasts which were treated for longer time and with higher concentration of MMC showed more suppression in growth rate. These results suggest that intraoperative application of MMC during dacryocystirhinostomy could have a positive effect of mucosal ostium with suppression of osteoblasts proliferation.
Key Words: Dacryocystorhinostomy;Mitomycin-C;Osteoblast


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