| Histologic Study after 5-Fluorouracil Injection into the Bleb in Ahmed Valve Implanted Rabbit Eyes. |
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Jeong Yoon Son, Seh Kwang Park, Young Il Kim |
1Department of Ophthalmology, In Je University College of Medicine, Korea. ytmns@yahoo.co.kr
2Balgeunsesang Eye Center, Pusan, Korea. |
| 가토안에서 아메드밸브삽입과 5-FU주입에 의한 여과포 조직변화 |
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손정윤 ( Son Jeong Yun ) , 박세광 ( Park Se Gwang ) , 김영일 ( Kim Yeong Il ) |
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| Abstract |
PURPOSE
To study the effect of the 5-fluorouracil (5-FU) injection into the bleb after the Ahmed glaucoma valve implant in rabbits. METHODS: Ahmed valve was implanted in normal 10 rabbits without previous ophthalmic history. In 4 of 10 rabbits, 5-FU (50 mg/ml, 0.1ml) was injected into filtering bleb in every two weeks for two times at postoperative 1 month and others were followed up as a control group. We obtained histological findings in 2 rabbis of control group at postoperative 1 month. In the control group and 5-FU injected 4 rabbit eyes, each 2 rabbit eyes were enucleated at postoperative 3 months and 6 months in both groups. The dissected bleb was examined through light microscopy with hematoxyline and eosin (H and E) stain and Masson-Trichrome (M-T) stain, and electron microscopy. RESULTS: The bleb was relatively avascular on gross and histological examination in the 5-FU injected group, and collagen fibers of the 5-FU injected group were less compact than that of the control group at postopeative 3 months. The number of inflammatory cells, fibroblasts and capillaris in outer layer was decreased in the 5-FU injected group. Under the electron microscopic examination, the density of collagen fibers was decreased and the space among them was widened. This change was more prominent in the 5-FU injected group. CONCLUSIONS: We found that the histological changes of blebs were made by the influx of aqueous humor through the Ahmed glaucoma valve and more stimulated by injection of 5-FU into the bleb after implantation of Ahmed glaucoma valve in a rabbit model. |
| Key Words:
Ahmed glaucoma valve;5-Fluorouracil;Rabbit eyes |
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