Effects of Filtering Surgery on the Level of MMP-2 in Rabbit.

Lee, Kyoung Min; Hong, Young Jae; Kim, Chan Yun

Original Article

Journal of the Korean Ophthalmological Society 2003;44(10):2417-2422.
Published online October 1, 2003.

Effects of Filtering Surgery on the Level of MMP-2 in Rabbit.

Kyoung Min Lee, Young Jae Hong, Chan Yun Kim

The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. KCYEYE@yumc.yonsei.ac.kr

가토에서 여과수술이 Matrixmetalloproteinase-2 (MMP-2) 발현에 미치는 영향

이경민 ( Lee Gyeong Min ) , 홍영재 ( Hong Yeong Jae ) , 김찬윤 ( Kim Chan Yun )

Abstract

PURPOSE
We investigated the effect of lowering of intraocular pressure by the filtering surgery on the expression of matrix metalloproteinase-2 of aqueous humor and trabecular meshwork. METHODS: Thirty-six white rabbits were used. Penetrating filtering surgery was performed in the one eye of each of eighteen rabbits and anterior chamber paracentesis and suture through sclera were performed on one eye of each eighteen rabbits as control group. The levels of MMP-2 in the aqueous sampling in two groups were checked by gelatin zymogram and Western blot, and the levels of MMP-2 in the trabecular cell extraction were checked by Western blots. RESULTS: The penetrating filtering surgery performed eye showed significant IOP depression than control group at post op 1 and 2 weeks (P<0.05). The penetrating filtering surgery performed eye showed more increased expression of MMP-2 in the aqueous humor in the zymogram at 2 and 3 weeks, and more increased expression of MMP-2 in the aqueous humor in the Western blot at 1 and 4 weeks. The penetrating filtering surgery performed eye showed more decreased expression of MMP-2 in the trabecula at post op 1 and 4 weeks. But these results were not statistically significant (P>0.05). CONCLUSIONS: The lowering of intraocular pressure by filtrating surgery showed no significant effect on the expression of MMP-2 of aqueous humor and trabecular meshwork.

Key Words: Anterior chamber;MMP-2;Rabbit