| Biochemical Analysis of Inter-Connection between Types of Nuclear Opacity, Oxidative Stress and Glycation in Cataractous Lens. |
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Kwang Won Lee, Jang Hyun Kim, Du Sik Shin, Jung A Kim, Sung Kun Chung, Jin Hak Lee, Choun Ki Joo |
1Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.
2Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea. |
| 백내장 수정체의 핵형 혼탁정도 , 산화 스트레스와 Glycation 반응의 상호 관련성에 관한 생화학적 분석 |
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정성근(Sung Kun Chung),김정아(Jung A Kim),주천기(Choun Ki Joo),이진학(Jin Hak Lee),이광원(Kwang Won Lee),김장현(Jang Hyun Kim),신두식(Du Sik Shin) |
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| Abstract |
PURPOSE
To elucidate the inter-connection between lens nuclear opacity and its proposed two main factors, which are oxidative stress and glycation reaction initiated with sugars and proteins. METHODS: We have collected clinical samples after cataract surgery from 57 patients during 3 month period. We also classified these samples into three groups as initial, intermediate and advanced stages, and performed the biochemical assays for measurements of glutathione(GSH) and advanced glycation end products(AGEs). RESULTS: It showed that the concentrations of GSH in lens soluble proteins were 0.022+/-0.019, 0.017+/-0.011, and 0.011+/-0.010 nmoles/mg lens protein in initial stage, intermediate stage and advanced stage, respectively. Fluorophore formation(Ex370/Em440), which has been reported to represent general AGEs formation, was positively correlated with nuclear opacity showing that 108.8+/-48.3, 174.7+/-116.9, and 188.2+/-130.6 Fluorescence Unit(F.U.) in initial stage, intermediate stage and advanced stage, respectively. Likewise, a marker for AGEs, pentosidine-related fluorophore(Ex335/Em385) was increased with nuclear opacity(67.0+/-30.8, 75.5+/-36.4, and 80.4+/-41.7 F.U. in initial stage, intermediate stage and advanced stage, respectively), although significant differences existed between groups. CONCLUSION: Our results showed that there are clear inter-connection between lens nuclear opacity, oxidative stress and the formation of general AGEs compared with pentosidine, and suggested that therapy using antioxidants, chelating agents, or glycation inhibitors could be beneficial in delaying nuclear cataractogenesis. |
| Key Words:
AGEs;Glycation;Nuclear cataract;Opacity;Oxidative stress |
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