Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis.

Lee, Sung Jin; Choi, You Jeong; You, Yong Sung; Koh, Hyoung Jun; Lee, Joon H; Lee, Sung Chul; Park, Jeon Han; Kwon, Oh Woong

Original Article

Journal of the Korean Ophthalmological Society 2001;42(4):600-606.
Published online April 1, 2001.

Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis.

Sung Jin Lee, You Jeong Choi, Yong Sung You, Hyoung Jun Koh, Joon H Lee, Sung Chul Lee, Jeon Han Park, Oh Woong Kwon

¹Department of Ophthalmology, Soonchunhyang University, College of Medicine.
²Department of Microbiology, Yonsei University College of Medicine.
³Department of Ophthalmology and The Institute of Vision Research, Yonsei University, College of Medicine.

Polymerase Chain Reaction 을 이용한 감염성 안내염의 진단

이성진(Sung Jin Lee),최유정(You Jeong Choi),유용성(Yong Sung You),고형준(Hyoung Jun Kob),이준행(Joon H Lee),이성철(Sung Chul Lee),박전한(Jeon Han Park),권오웅(Oh Woong Kwon)

Abstract

PURPOSE
We evaluated the efficacy of the PCR which is known as more sensitive method than culture in the diagnosis of causal microorganisms of the infective endophthalmitis. METHODS: We used 0.3 ml of aqueous humor and 0.5 ml of vitreous sample in 3 cases of postoperative and 1 case of endogenous endophthalmitis for detecting causal microorganisms. To detect the bacteria we used universal, Gram positive and negative primers, and to detect the fungus we used fungal primer. RESULTS: Three cases of endophthalmitis, there was no bacteria in the bacterial culture for 10 days but PCR results identified causal microorganisms in all cases. CONCLUSIONS: PCR is effective in the fast and accurate diagnosis of infective endophthalmitis and especially in the culture-negative endophthalmitis.

Key Words: Culture;Diagnosis;Endophthalmitis;PCR(polymerase chain reaction)