| Ocular Penetration of Topical Ofloxacin in Rabbit Eye with Amniotic Membrane Transplantation. |
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Hyun Seung Kim, Jung Il Moon, Woo Jin Sah, Young Jin Kim, Jae Chan Kim, Tae Won Hahn |
1Department of Ophthalmology, College of Medicine, The Catholic University of Korea.
2Department of Ophthalmology, College of Medicine, The Chungang University, Seoul, Korea.
3St. Mary's Eye Center. |
| 토끼 눈에서 양막이식이 Ofloxacin 의 안내 침투에 미치는 영향 |
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김재찬(Jae Chan Kim),김현승(Hyun Seung Kim),문정일(Jung Il Moon),사우진(Woo Jin Sah),김영진(Young Jin Kim),한태원(Tae Won Hahn) |
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| Abstract |
PURPOSE
We evaluated ocular penetration and drug level in tear after topical ofloxacin instillation in rabbit eye with amniotic membrane transplantation(AMT). METHODS: In the first set of experiment, 24 rabbits(24 eyes) were divided into 4 groups according to the epithelial removal or AMT. Topical ofloxacin was instilled 4 times every 15 minutes and then, 1 hour after the last eyedrop, the samples of amniotic membrane(AM), cornea and aqueous humor were collected. In the second set of experiment, 24 rabbits(24 eyes) were divided into 6 groups according to the freshness of AM or its attached time. Topical ofloxacin was applied to eyes and then, tear samples were collected at 30 minutes, 1, 2, 4, and 6 hours. Ofloxacin concentration in the samples of two experiments were evaluated using high performance liquid chromatography. RESULTS: Mean ofloxacin concentrations in cornea and aqueous humor were statistically higher in deepithelized corneas regardless of AMT(P<0.05). And mean tear levels of ofloxacin in AMT groups were statistically higher than those in non-AMT groups(P<0.05). CONCLUSIONS: AMT seems to interfere with the ocular penetration of topical ofloxacin in normal rabbit cornea, but rather enhances ofloxacin penetration in the cornea with epithelial defect. And also the ofloxacin level in tear was higher in eyes with AMT up to 1 hour after topical ofloxacin use. Therefore it seems that AM has potential to act as an effective drug delivery system. |
| Key Words:
Amniotic membrane;Drug delivery;High performance liquid chromatography;Ocular |
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