Histochemical Electron Microscopic Findings of the Distribution of the Glycosaminoglycans of Cultured Sensory Retina.

Kim, Jin Sang; Kwon, Ji Won; Ko, Myung Kyoo

Original Article

Journal of the Korean Ophthalmological Society 2002;43(1):197-203.
Published online January 1, 2002.

Histochemical Electron Microscopic Findings of the Distribution of the Glycosaminoglycans of Cultured Sensory Retina.

Jin Sang Kim, Ji Won Kwon, Myung Kyoo Ko

¹Department of Ophthalmology, College of Medicine, Hanyang University Hospital, Korea. fovea@hanyang.ac.kr
²Department of Ophthalmology, College of Medicine, University of Ulsan, Asan Medical Center, Korea.

배양된 신경망막에서 Glycosaminoglycans의 분포에 대한 조직화학 전자현미경 소견

김진상 ( Jin Sang Kim ) , 권지원 ( Ji Won Kwon ) , 고명규 ( Myung Kyoo Ko )

Abstract

PURPOSE
This study was performed to investigate the distribution of glycosaminiglycans by the activated retinal neuronal cell on the cultured retinal tissue. METHODS: The retinal tissue was obtained from the donor eyeball without the pathological findings of the retina. The dissected retinal tissue was cultured for 2 weeks in the culture media and reacted with ruthenium red dye to observe the glycosaminoglycans reaction with transmission electron microscopy. RESULTS: The histochemical reaction to ruthenium red on the cultured retina tissue was prominent on the internal limiting membrane, intercellular space between the axons and dendrites at the outer and inner plexiform layers and interphotoreceptor matrix. CONCLUSIONS: These findings suggest that GAGs may be released into the extracellular spaces between the axons and dendrites, which modulate the retinal circuits.

Key Words: Culture;Electron microscopy;Glycosaminoglycans;Retina;Ruthenium red