| Antioxidant Assay of Extracted Fractions Xanthium Strumarium L Using Lens Protein Crosslink Activity. |
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Seok Jong Lee, Kwang Won Lee, Young Shin Chung, Eun Kyung Hong, Jin Hak Lee, Won Rayng Wee, Choun Ki Joo |
1Laboratory of Ophthalmology and Visual Science, College of Medicine, The Catholic university of Korea, Seoul, Korea. ckjoo@cmc.cuk.ac.kr
2Medvill.Co.Ltd, Korea.
3Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea. |
| 수정체 단백질 Crosslink 활성을 이용하여 창이자로부터 추출된 분획물들의 항산화효과 측정 |
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이석종 ( Seok Jong Lee ) , 이광원 ( Kwang Won Lee ) , 정영신 ( Young Shin Chung ) , 홍은경 ( Eun Kyung Hong ) , 이진학 ( Jin Hak Lee ) , 위원량 ( Won Rayng Wee ) , 주천기 ( Choun Ki Joo ) |
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| Abstract |
PURPOSE
To elucidate the anti-oxidant effect of extract fractions from Xanthium strumarium L. on lens protein by crosslinking assay. METHODS: [(1 4)C] N-formyl-lysine was synthesized and purified by ion exchange chromatography. The crosslinking activities of extract fractions(Xan Crude, Xan CHCl3, Xan EtAc and Xan H2O) to lens protein were determined by incorporation with [(14)C] N-formyl-lysine. RESULTS: It was observed that Xan Crude, Xan CHCl3, and Xan EtAc extracted from Xanthium strumarium L. showed approximately 10% of antioxidant effect whereas Xan H2O showed no effect by crosslinking assay. CONCLUSIONS: This study showed that the crosslinking assay described in this study can be developed as a potential tool to screen the anti-oxidant effect rapidly and accurately compared to MTT assay. The result was compared to MTT assay using Human Lens epithelial cell line. |
| Key Words:
Cataract;Crosslink;Glycation;Antioxidant |
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