| Effects of Corneal Neovascularization on TNF-alpha and TGF-beta 2 mRNA Expression in Endotoxin Induced Uveitis. |
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Sun Ju Choi, Jong Wook Kim, Jong Woo Kim, Joo Young Park |
1Department of Microbiology, Wonju College of medicine, Yonsei University, Wonju, Korea.
2Department of Ophthalmology, Myung Gog Eye Research Institute, College of medicine, Konyang University, Daejon, Korea. kjwook@kyuh.co.kr |
| 각막신생혈관이 내독소에 의한 포도막염에서 TNF-α, TGF-β2 mRNA의 발현에 미치는 영향 |
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최선주 ( Sun Ju Choi ) , 김종욱 ( Jong Wook Kim ) , 김종우 ( Jong Woo Kim ) , 박주영 ( Joo Young Park ) |
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| Abstract |
PURPOSE
We tried to evaluate the expression of TNF-alpha and TGF-beta 2 mRNA during the course of the endotoxin induced uveitis (EIU) in rat after inducing neovascularization on cornea. METHODS: Reverse transcription followed by polymerase chain reaction amplification was used to determine relative mRNA level in two ocular tissues (iris-ciliary body, cornea) at 0, 1, 3, 6, 12, 48 hours after subcutaneous injection of 200 ug of Escherichia coli endotoxin. RESULT: Rat with corneal neovascularization showed somewhat different pattern of expression. TGF-beta 2 mRNA expression was showed some fluctuations in cornea. TGF-beta 2 mRNA expression in cornea was decreased within 3 hours after endotoxin treatment and increased gradually after 6 hours until 48 hours. CONCLUSION: TGF-beta2 might be one of the important cytokines in EIU, especially animals with neovascularization on cornea. |
| Key Words:
Endotoxin induced uveitis;Endotoxin;TNF-alpha;TGF-beta2 |
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