| The Effects of Nitric Oxide Synthesis and Cellular Metabolic Activity by Glucose, Interleukine-1beta, and Transforming Growth Factor-beta1 in Human Umbilical Vascular Endothelial Cells. |
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J S Lee, B S Oum, C H Park, Y S Kim |
1Department of Ophthalmology, Pusan National University Medical College.
2Dr. Park`s Eye Clinic.
3Medical Research Institute, Pusan National University Hospital. |
| 혈관내피세포에서 Glucose , Interleukine - 1β , Transforming Growth Factor - β1 이 Nitric Oxide 생성 및 세포대사작용에 미치는 영향 |
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이종수(Jong Soo Lee),엄부섭(Boo Sup Oum),박창호(Chang Ho Park),김유선(Yoo Sun Kim) |
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| Abstract |
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The authors studied the effects of NO synthesis, cellular metabolic activity, and cellular morphology in the human umbilical vascular endothelial cells[HUVECs] by the concentration of glucose and cytokines such as interleukine[IL]-1 and transfoming growth factor[TGF]-beta1. A high concentration of glucose slightly increased NO synthesis, and a higher concentration of TGF-beta1 decreases NO synthesis in a concentration-dependent manner. Especially, the effect on NO synthesis of glucose or cytokine was distinctly revealed in a culture medium with fetus bovine serum. In cellular viability, there was no significant difference among glucose, IL-1, TGF-beta1 and both IL-1 and TGF-beta1 combined, when compared to the control. The typical cells as well as cells stimulated by IL-1 have relatively round spindle shape, but the cells stimulated by TGF-beta1 have a more elongated spindle shape. In ultrastructual findings, normal cells had slightly dilatated rough endoplasmic reticulum, mitochondria, and golgi complex. However, the cells stimulated by TGF-beta1 showed an atypical ultrastructual evidence of some bundles of microfilaments and dense plaque-like materials in the cytoplasm. As glucose or various kinds of cytokines can induce NO synthesis and cellular differentiation, it is very important that the control of glucose or cytokine in vascular diseases can inhibit the synthesis of free radicals and cellular differentiation of vascular endothelial cells. Furthermore, it is desirable to study the role of cellular differentiation in vascular endothelial cells stimulated by TGF-beta1. |
| Key Words:
Cytokine;Glucose;Interleukin-1;Nitric oxide;Transforming growth factor-beta1 |
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