An Experimental Study of the Effect of TGF-beta1 on the Growth and Apoptosis in Y-79 Retinoblastoma Cells.

Lee, M A; Kwak, H W; Jin, K H; Kim, J M

Original Article

Journal of the Korean Ophthalmological Society 1999;40(12):3368-3376.
Published online December 1, 1999.

An Experimental Study of the Effect of TGF-beta1 on the Growth and Apoptosis in Y-79 Retinoblastoma Cells.

¹Department of Ophthalmology, Andong General Hospital.
²Department of Ophthalmology, College of Medicine, Kyung Hee University, Seoul, Korea.

Y - 79 망막모세포종 세포의 성장과 고사에 미치는 TGF - β1 의 영향에 관한 실험적 연구

김재명(Jae Myung Kim),이미애(Mi Ae Lee),곽형우(Hyung Woo Kwak),진경현(Kyung Hyun Jin)

Abstract

The effect of TGF-beta1 on the growth of Y-79 retinoblastoma cells was examined. Y-79 retinoblastoma cells treated with TGF-beta1[0.5 ng/ml, 1.0ng/ml] were cultured and their growth curve was compared to the untreated cultured cells. Phase contrast microscopy, transmission electron microscopy and flow cytometer were also performed. Administration of TGF-beta1 significantly inhibited growth of Y-79 cells[p<0.05]. Transmission electron microscopic examination of those cells showed nuclear condensation, fragmentation and cytoplasmic blebbing, suggesting apoptosis as an underlying mechanism. And apoptosis was quantified by flow cytometric analysis. TGF-beta1 significantly induced apoptosis in Y-79 cells[p<0.05]. The histogram of TGF-beta1 treated Y-79 cells showed an increase in G2/M populations and decrease in G1 cell populations. This study suggested that TGF-beta1 induced apoptosis in Y-79 retinoblastoma cells, and the most plausible mechanism of growth inhibition and this function might be related to G2/M rather than G1 of the cell cycle related to Rb protein.

Key Words: Apoptosis;Retinoblastoma cells;TGF-beta1