Apoptotic Cell Death in Experimental Retinal Vein Occlusion.

Kang, Shin Goo; Chung, Hum

Original Article

Journal of the Korean Ophthalmological Society 2000;41(3):576-584.
Published online March 1, 2000.

Apoptotic Cell Death in Experimental Retinal Vein Occlusion.

¹Department of Ophthalmology, College of Medicine, Seoul National University.
²Department of Ophthalmology, Eulji University School of Medicine, Tajeon, Korea.

망막정맥폐쇄 동물모델에서 아포프토시스의 발현

정흠(Hum Chung),강신구(Shin Goo Kang)

Abstract

We used an animal model of laser-induced retinal vein occlusion to study the temporal and spatial patterns of neuronal necrosis and apoptosis. After photodynamic retinal vein thrombosis with argon-green laser, rats were sacrificed at 6 hours, 12 hours, 1 day, 2 days, 3 days, 5 days, 7 days, and 14 days after vein occlusion. The temporal and spatial patterns of neuronal cell death were determined using light and electron microscopy, TdT-dUTP nick-end labeling[TUNEL]and DNA gel electrophoresis. The cells in retinal ganglion cell layer and inner nuclear layer showed both necrotic and apoptotic changes 12 hours after vein occlusion. The TUNEL positivity were detected at day 1 after vein occlusion and the number of positive cell increased until day 2, and decreased thereafter. DNA ladder pattern was observed 48 hours after vein occlusion by DNA gel electrophoreisis. These data demonstrated that retinal vein occlusion induces necrosis and apoptosis in the retinal ganglion cell and inner nuclear layer.

Key Words: Apoptosis;Ischemic injury;Retinal ganglion cell;Retinal vein occlusion;TUNEL staining