The Role of L-ascorbate on Cell Death Induced by Ultraviolet A in Ciliary Body Epithelium.

Ahn, Byoung Sun; Park, Hyung Nam; Kee, Changwon

Original Article

Journal of the Korean Ophthalmological Society 2000;41(4):847-854.
Published online April 1, 2000.

The Role of L-ascorbate on Cell Death Induced by Ultraviolet A in Ciliary Body Epithelium.

Byoung Sun Ahn, Hyung Nam Park, Changwon Kee

¹Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
²Samsung Biomedical Research Institute.

자외선 A 에 의한 모양체 세포의 세포사에 있어 L - Ascorbate 의 역할

안병선(Byoung Sun Ahn),박형남(Hyung Nam Park),기창원(Chang Won Kee)

Abstract

To evaluate the effect of ascorbate on cell death induced by ultraviolet A, cultured human ciliary body epithelial cells were exposed the UV-A at energy level of 10 and 30J/cm2. Before and after UV-A exposure the experimental group was treated with 250 microgram/ml L-ascorbate while the control group was not. Cell viability was 87.3% in the control group and 97.1% in the experimental group at energy level of 10J/cm2UV-A. Cell viability was decreased to 38.8% in the control group and 73.5% in the experimental group at energy level of 30J/cm2 UV-A. The cells in the experimental group were less affected. This phenomenon was also observed by light microscope. The degree of apoptosis was 4.68% in the control group and 4.11% in the experimental group at the level of 10J/cm2 UV-A. The apoptosis was increased to 11.01% in the control group and 4.16% in the experimental group at the level of 30J/cm2 UV-A. On electron microscope necrosis and apoptosis induced by UV-A were observed in both groups but the degree of cell death was much less in the experimental group. In conclusion, L-ascorbate has a role of decreasing cell death induced by UV-A in ciliary body epithelial cells.

Key Words: Apoptosis;Ciliary body epithelial cells;L-ascorbate;Necrosis;UV-A