| The Minimal Inhibitory Concentrations of Antifungal Agents for Clinical Isolates in Fungal Keratitis. |
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Seung Wan Sohn, Young Ho Hahn |
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Department of Ophthalmology, Gospel Hospital, The Kosin University, Pusan, Korea. |
| 진균각막염의 원인균주에 대한 항진균제감수성검사 |
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한영호(Young Ho Hahn),손승완(Seung Wan Sohn) |
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| Abstract |
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This study was performed for the standardization and proper selection of effective antifungal agents by measuring the minimal inhibitory concentra-tions[MICs]of antifungals to fungi, separated from keratitis patients.Two strains of A.fumigatus and single strain of F.solani, A.falciforme, and A . alternata were used for this test.Standard powders of miconazole, itraconazole, clotrimazole, ketoconazole, and amphotericin B were used as antifungal agents. Microscopic and macroscopic measurements of MIC after 24, 48 and 72 hours of inoculation[105 conidia /ml]into YNB broth with culture temperature of 25 degrees C were performed by use of broth microdilution method.The results are as follows : itraconazole, amphotericin B, and clotrimazole were effective against A.fumigatus.F.solani showed resistance to all kinds of antifungal agents.A.falciforme and A.alternata were sensitive to amphotericin B and itraconazole, respectively. Further studies may be needed for the standardized measurement of MIC against filamentous fungi and also for in Vitro-in Vivo correlations for the treatment of fungal keratitis. |
| Key Words:
Antifungal agent;Fungal keratitis;Microscopic minimal inhibitory concentration[MIC];Susceptibility test |
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