Carbonic Anhyd rase Activity in Muller Cell.

Cho, Nam Chun; Kweon, Eui Yong

Original Article

Journal of the Korean Ophthalmological Society 2000;41(5):1068-1072.
Published online May 1, 2000.

Carbonic Anhyd rase Activity in Muller Cell.

Nam Chun Cho, Eui Yong Kweon

Department of Ophthalmology, College of Medicine, Chonbuk National University, Korea.

뭘러세포의 탄산탈수효소 분포

조남천(Nam Chun Cho),권의용(Eui Yong Kweon)

Abstract

Carbonic anhydrase, an enzyme catalysing the reversible hydration of carbon dioxide, is present in the Muller cells.Because the enzyme is not present in other uroretinal cells in the retina, it can be used as a marker for Muller cells.Carbonic anhydrase activity was demonstrated bnzymehistochemically in human and rabbit Muller cells to know a relation of metabolic functions and carbonic anhydrase activity.Human retinas were obtained from donor eyes.The eyes were enucleated immediately after death forenzymatic activity. In human retina, heavy staining was found in the inner nuclear layer and nerve fiber layer, moderate staining in the outer nuclear layer and weak or no staining in the plexiform layers.In rabbit retina, heavy staining was found in the nerve fiber layer and nuclear layers and weak reaction in the two plexiform layers. These findings suggest that Muller cells may participate in CO2 homeostasis mechanism of carbonic anhydrase in the retina.

Key Words: Carbonic anhydrase;Muller cell;Metabolism