| Fluorophotometric Detection of Oxygen Free Radicals in the Cornea and Lens after Excimer Laser Photore fractive Keratecto my in Rabbit. |
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Jun Ho Heo, Won Ryang Wee, Jin Hak Lee |
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Department of Ophthalmology, Seoul National University, College of Medicine, Seoul, Korea. |
| 토끼에서 엑시머레이저 굴절교정각막절제술 후 발생한 각막 및 수정체 내 산소유리기의 형광측정기를 이용한 측정 |
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허준호(Jun Ho Heo),위원량(Won Ryang Woo),이진학(Jin Hak Lee) |
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| Abstract |
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This study was performed to detect the oxidative stress in the cornea and lens in vivo by fluorophotometer after excimer laser photorefractive keratectomy(PRK). Twenty New Zealand white rabbits(20 eyes)were divided into two groups, control group(10 eyes)and PRK group(10 eyes). Rabbits of PRK group underwent PRK(8 diopters)and 2', 7'-dichlorofluorescein diacetate(DCFH-DA)was applied on the central cornea 6 hours after PRK in both groups. Fluorophotometric readings of the cornea and lens were measured 15 minutes(F15)and 30 minutes(F30)after DCFH-DA application in both groups. The cornea and lens was observed with slit lamp biomicroscope for 8 weeks. F15 and F30 of the cornea in PRK group(3.83+/-1.22 ng/ml, 5.12+/-1.57 ng/ml)were significantly higher than those in control group(2.61+/-0.59 ng/ml Eq, 3.26+/-0.76 ng/ml Eq)(p=0.01, p=0.005). F15 and F30 of the lens in PRK group(409.7+/-157.2 ng/ml, 594.9+/-242.2 ng/ml Eq)were significantly higher than those in control group(74.1+/-48.3 ng/ml Eq, 310.1+/-249.8 ng/ml Eq)(p=0.0004, p=0.01). No lens opacity developed and F15 and F30 of the cornea did not correlate with corneal haze determined at postoperative 8 weeks. In this study, we demonstrated increased oxygen free radicals in the cornea and lens after PRK by fluorophotometer in vivo and this method can be used as a useful tool to investigate the possible damage induced by oxygen free radicals in the cornea and lens after excimer laser corneal ablation. |
| Key Words:
Cornea;Excimer laser photorefractive keratectomy;Fluo-rophotometer;Lens;Oxygen free radicals;2';7'-dichlorofluo-rescein diacetate |
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