The Expression of IL-1 and TNF-alpham RNA in Alkali-burned Cornea in Rabbits.

Kim, Dong Gun; Park, Jong Moon

Original Article

Journal of the Korean Ophthalmological Society 2000;41(10):2044-2050.
Published online October 1, 2000.

The Expression of IL-1 and TNF-alpham RNA in Alkali-burned Cornea in Rabbits.

Department of Ophthalmology, College of Medicine, Gyeongsang National University, #92 Chilam-dong, Chinju, Gyeongsangnamdo, 660-280, Korea.

백색가토의 각막에서 알카리 각막화상에 의한 IL - 1 과 TNF - α mRNA 의 발현

김동건(Dong Gun Kim),박종문(Jong Moon Park)

Abstract

This study investigated the cytokines (IL-1alpha, IL-1beta, IL-1RA, TNF-alpha) mRNA expression in the early inflammatory process of alkali burned rabbit corneas. To create alkali burns, each 4 cornea of New Zealand White rabbits were exposed to filtering paper (5 mm diameter) treated with 1N NaOH for 1 minute at room temperature.e. Two unburned rabbits corneas were used as control. The mRNA levels for IL-1alpha, IL-1beta, IL-1RA, and TNF-alpha were monitored in corneas at 3, 6, 24, and 48 hours after alkali burn with semiquantative reverse transcription-polymerase chain reaction. Transcripts for IL-1alpha, IL-1beta, and TNF-alphawere expressed in 3 hours and increased through 48 hours, whereas those for IL-1betawas expressed in 3 hours and was not changed through 48 hours. IL-1alphamRNA level was detected lower than IL-1betamRNA in 3 hours but IL-1alphamRNA level was persist higher than IL-1betafrom 6 hours through 48 hours. IL-1RA mRNA level was expressed higher than others through 48 hours. These results indicate that IL-1 and TNF-alphalevel in the cornea are elevated during the early inflammatory process of alkali burn. IL-1alpha, IL-RA and TNF-alphamay play an important role in alkali-burned corneal inflammatory process.

Key Words: IL-1;TNF-alpha;Reverse transcription-polymerase chain reaction