Protective Effect of Thiore doxin on Bovine Corneal Endothelial Cells Damaged by Oxidative Stresses.

Lee, Dong Yeon; Lee, Yun Song

Original Article

Journal of the Korean Ophthalmological Society 2000;41(10):2060-2068.
Published online October 1, 2000.

Protective Effect of Thiore doxin on Bovine Corneal Endothelial Cells Damaged by Oxidative Stresses.

¹Department of Ophthalmology, Pundang Jesaeng General Hospital, Sungnam, Korea.
²Department of Pharmachology, Sungkwunkwon University School of Medicine, #300 Cheoncheon-dong, Jangan-ku, Suwon, 440-746, Korea. yslee@yurim.skku.ac.kr

소의 각막내피세포의 산화적 손상에 대한 Thioredoxin 의 보호효과

이동연(Dong Yeon Lee),이윤송(Yun Song Lee)

Abstract

Reactive oxygen species including hydrogen peroxide are known to induce morphological and functional derangement of corneal endothelium, thus causing loss of corneal deturgescence. Among the antioxidants, thioredoxin has been recently suggested to play an important role under oxidative stresses. The present study was undertaken to investigate the protective effect of thioredoxin, obtained from Escherichia coli, on cultured bovine endothelial cells against hydrogen peroxide and ultraviolet ray. Thioredoxin at concentrations of 1, 10 and 100 microgram/ml appeared to protect corneal endothelial cells from the reactive oxygen radical hydrogen peroxide as assessed by measuring the leakage of lactate dehydrogenase (LDH) and the reduction of 3-[4, 5-dimethyl-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT). In conclusion, thioredoxin may play an important physiological role (s) in protection of corneal endothelial cells which is continuously challenged by reactive oxygen species. Thus, It is natural that thioredoxin may have clinical implications in certain corneal disease such as corneal edema, which is known to be caused by reactive oxygen species.

Key Words: Reactive oxygen species;Thioredoxin;Corneal endothelial cell