Rat Cornea Model for Inhibition of Angiogenesis Induced by Suture.

Park, Ji Young; Chung, Sung Kun; Joo, Choun Ki

Original Article

Journal of the Korean Ophthalmological Society 1998;39(12):2867-2874.
Published online December 1, 1998.

Rat Cornea Model for Inhibition of Angiogenesis Induced by Suture.

Ji Young Park, Sung Kun Chung, Choun Ki Joo

Department of Ophthalmology, The Catholic University Medical College, Catholic Research Institutes of Medical Science.

봉합으로 유도한 신생혈관을 억제하기 위한 흰쥐 각막모델

박지영(Ji Young Park),정성근(Sung Kun Chung),주천기(Choun Ku Joo)

Abstract

The purpose of this study was to establish a model system of induced neovascularization(NV) and the inhibitory chemicals tested on the avascular tissue, namely cornea. We induced corneal NV condition by suturing the cornea of Sparague-Dawley rat with one stitch of 10-0 nylon in immitation of corneal transplantation and terramycin was applied on the cornea for 1 week to prevent corneal inflammation. The progression of suture-induced corneal NV was investigated at various time pints(1, 7 days, 2, 3, 4 and 8wks) after the suture. The formation of new capillary tubs was observed at 5-7 days after suture. The arc of NV was increased gradually and peaked in 3 weeks and decreased hereafter while the length of vessels did not change during he course of NV once it reached the maximum. We also developed a corneal model for NV inhibition in which CB 2`-ph(1)(2`-O-Benzonylcinnamaldehyde), the NV inhibitor, contained in the form of Hydron Pellet was inserted into the stroma of the cornea followed by suturing the cornea. In this model for inhibition of NV, Hydron Pellet was inserted in the cornea successfully without causing the corneal inflammation. In this study, we developed a suture-induced corneal NV model system which enables us to perform various experiments reproducibly and investigate the effect of NV inhibitor.

Key Words: 2`-O-Benzonylcinnamaldehyde;Corneal Angiogenesis;Suture