The Effect of Intravitreal Injection of Retinoic Acid on Experimentally Induced Proliferative Vitreoretinopathy using Human Retinal Prgment Epithelium in Rabbits.

Kim, Min Seop; Yang, Han Nam

Original Article

Journal of the Korean Ophthalmological Society 1999;40(1):146-153.
Published online January 1, 1999.

The Effect of Intravitreal Injection of Retinoic Acid on Experimentally Induced Proliferative Vitreoretinopathy using Human Retinal Prgment Epithelium in Rabbits.

Min Seop Kim, Han Nam Yang

Department of Ophthalmology Kangbuk Samsung Hospital, College of Medicine, Sung Kyun Kwan University.

토끼에서 사람망막색소상피세포를 이용하여 유발시킨 증식유리체망막병증에서 유리체강내 투여한 Retinoic Acid의 효과

김민섭(Min Seop Kim),양한남(Han Nam Yang)

Abstract

The retinoic acid have been demonstrated to modulate the growth and differentiation of several cell types including retinal pigment epithelium.We evaluated the effect of intravitreal infection of retinoic acid on experimentally induced PVR using human retinal pigment epithelium in the rabbit. 35 eyes of rabbit were induced PVR by injecting of human retinal pigment epithelial cells and were divided into three groups; Control group, group 1(retinoic acid 0.05mg/0.1ml DMSO was injected) and group 2(retinoic acid 0.1mg/0.1ml DMSO was injected). The stages of PVR were observed on days 3, 7, 14 and 28. The mean PVR stage progressed in all three groups. The PVR stage in control group is lower than other groups on days 3 and the PVR stage in group 3 is higher than other groups on days 28. In conclusions, intravitreal injected retinoic acid cannot inhibit PVR proliferation on 0.05 and mg/0.1ml concentration and it had toxic effect on retina in 0.1mg/0.1ml concentration. This results may present the lower limit of concentration to use retinoic acid intravitreously.

Key Words: Experimentally induced proliferative vitreoretinopathy;Human retinal pigment epithelium, Intravitreal injection;Retinoic acid