Inhibition of Basic Fibroblast Growth Factor Induced Corneal Angiogenesis by a Farnesyl Transferase Inhibitor.

Chung, Sung Kun; Lee, Ja Young; Joo, Choun Ki; Kim, Jae Ho

Original Article

Journal of the Korean Ophthalmological Society 1999;40(3):657-661.
Published online March 1, 1999.

Inhibition of Basic Fibroblast Growth Factor Induced Corneal Angiogenesis by a Farnesyl Transferase Inhibitor.

Sung Kun Chung, Ja Young Lee, Choun Ki Joo, Jae Ho Kim

Department of Ophthalmology, School of Medicine, The Catholic University of Korea.

Basic Fibroblast Growth Factor에 의해 유도된 각막 혈관생성에 있어서 파네실 전달효소 저해제의 억제효과

김재호(Jae Ho Kim),이자영(Ja Young Lee),정성근(Sung Kun Chung),주천기(Choun Ki Joo)

Abstract

Farnesyl transferase inhibitors(FTI) disrupt fanesylation of ras protein and thus, suppress tumor growth in vivo, To determine whether FTI extracted from Cinnamomum Cassia Blume(CB2`-ph) interferes with angiogenesis, we studied the effect of CB2`-ph on rabbit corneal angiogenesis induced by basic fibroblast growth factor(bFGF). A hydrogel disk containing 1000ng of bFGF was implanted intrastromally in the superior cornea of 12 NZW rabbit eyes. All eyes received a second intrastromal disk, randomized to contain either 40 micro gram of CB2`-ph(n=6) or phosphate-buffered saline(PBS)(n=6). Both disks were positioned side-by-side, 1.2mm from the superior limbus. Each eye was examined daily by two masked of new blood vessels. At 3, 5, and 7 days postimplantation of bFGF disks, eyes treated with CB2`-ph showed mean angiogenesis score of 6.0 +/- 4.8, 25.6 +/- 23.9 and 38.1 +/- 28.3, respectively, while PBS-treated controls scored 10.4 +/- 9.2, 27.2 +/- 16.7, and 39.0 +/- 22.8, respectively(p>0.4, Wilcoxon signed rank test). In a rabbit corneal pocket assay, CB2`-ph appears to be ineffective against bFGF-induced corneal angiogenesis in the model.

Key Words: Basic fibroblast growth factor;Corneal pocket assay;Farnesyl transferase inhibitor