Effect of Trusopt and Xalatan on the Proliferation of Cultured Subconjunctival Fibro blasts.

Kim, Jae Woo; Kim, Eung Cheol; Lee, Seung Soo

Original Article

Journal of the Korean Ophthalmological Society 1999;40(6):1642-1648.
Published online June 1, 1999.

Effect of Trusopt and Xalatan on the Proliferation of Cultured Subconjunctival Fibro blasts.

Jae Woo Kim, Eung Cheol Kim, Seung Soo Lee

Department of Ophthalmology, College of Medicine, Catholic University of Taegu-Hyosung.

Trusopt와 Xalatan이 배양된 섬유아세포의 증식에 미치는 효과

김재우(Jae Woo Kim),김응철(Eung Cheol Kim),이승수(Seung Soo Lee)

Abstract

The prior long-term use of topical anti-glaucoma medications has been suggested as an adverse factor for the outcome of trabeculectomy. The mechanism of action is still unclear. This study investigates the effect of Trusopt and Xalatan on the proliferation and viability of human Tenon`s capsule fibroblasts in tissue culture. We examined the effect of two commercial drugs, Trusopt and Xalatan, and pure benzalkonium chloride, at various dilution, on the proliferation as assessed by MTT assay and assessed micro-scopically the viability of human Tenon's capsule fibroblasts in tissue culture. None of the tested compounds stimulated proliferation of fibroblasts in tissue culture and inhibited proliferation. All had toxic effects on cell morphology. These results show an antiproliferative and toxic effect of Trusopt and Xalatan on the fibroblasts in tissue culture and no direct stimulation of fibroblast proliferation.

Key Words: Culture;Fibroblast;Proliferation;Trusopt;Xalatan