| Lectin Histochemistry of the Mucin on Corneal Epithelial Surface in Rabbit. |
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박중원(Jung Won Park),김응권(Eung Kweon Kim),김수진(Su Jin Kim),강신정(Shin Jeong Kang),김형래(Hyung Lae Kim),임지현(Ji Hyun Lim) |
1Department of Ophtalmology, The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
2Department of Biology, Hallym University, Chuncheon, Korea.
3Department of Ophtalmology, Inje University Sanggye Paik Hospital, Seoul, Korea.
4Department of Biochemistry, Ewha Woman;s University College of Medicine, Seoul, Korea. |
| 가토 각막 표층 뮤신의 Lectin 염색에 의한 성분 분석 |
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박중원(Jung Won Park),김응권(Eung Kweon Kim),김수진(Su Jin Kim),강신정(Shin Jeong Kang),김형래(Hyung Lae Kim),임지현(Ji Hyun Lim) |
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| Abstract |
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The mucous layer located on the cornea epithelial surface is mainly com posed of mucin. It is unique in being composed of a central protein back bone surrounded by an outermost dense oligosaccharide matrix, yet its precise composition is still unknown. In order to contribute to an understanding of the poorly characterized mucin of the corneal epithelial surface, we investigated the distribution of specific sugar moieties, an important component of mucin, in rabbits using biotinylated-lectin complexes and inhibitory sugars with 11 different lectins(RCA-I, PHA-E, SNA, WGA, MAA, SBA, DBA, PNA, UEA-I, LCA, Con A). The corneas were fixated with 1% glutaraldehyde-1% paraformaldehyde for 24 hrs and then embedded in paraffin. Lectin (20 microgram/ml) staining was performed on 2 micromiter thickness sections for 8 hrs. RCA-I, PHA-E, SNA, WGA, MAA, and SBA reacted Intensely. DBA and PNA bound moderately, Con A and UEA-I bound weakly, and LCA bound only slightly to the surface of the corneal epithelium. The strong staining of RCA-I, PHA-E, SNA, WGA, MAA, and SBA showed that beta-D-Galactose, D-Galactose, D-N-acetyl-galactosamine, NeuAce (alpha2,6) Gal, NeuAce (alpha2,3) Gal, D-GalNAc, and beta-D-GlcNA are the main components of muffin on the corneal epithelium in rabbits. The authors' results showed that mucin on the corneal epithelium in rabbit is composed of complex glycoproteins. |
| Key Words:
Corneal epithelium;Lectin;Mucin |
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