Distribution of the Glycosaminoglycans in the Bruch`s Membrane and Sensory Retina : Histochemical Electromic roscopic Findings with Ruthenium Red. |
Won Ki Lee, Myung Kyoo Ko, Dong Seob Kim |
Department of Ophthalmology, Chung-Ang Gil Medical Center, College of Medicine, Gachon University, Inchon, Korea. |
브르크막 및 신경망막에서 Glycosaminoglycans의 분포:Ruthenium Red 염색에 의한 조직화학전자현미경 소견 |
이원기(Won Ki Lee),고명규(Myung Kyoo Ko),김동섭(Dong Seob Kim) |
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Abstract |
The distribution of glycosaminoglycans in Bruch`s membrane and sensory retina of human eye was evaluated with histochemical electron microscope using 0.05%ruthenium red (RR). Seven donated or enucleated eyeballs without chorioretinal disease were used.Electron-dense RR reactive materials were identified as fine discrete granules along the basement membrane of the retinal pigment epithelium, in the inner and outer collagen layer and central elastic layer of Bruch 's membrane, and in the interphotorecep- tor matrix. In addition, they were observed at the cytoplasmic wall of photoreceptor cells, their intercellular spaces, and the internal limiting membrane. These results demonstrated that GAGs are widely distributed within the interphotoreceptor matrix, Bruch`s membrane, and intercellular spaces of retinal neurons. They are considered to function as a mechanical supporter as well as a selective filtration barrier through which the metabolitespass. |
Key Words:
Glycosaminoglycan;Ruthenium red;Bruch`s membrane;retina;Ultrastructure |
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