The Effect of Mitomycine C (MMC) on Inhibition of Pterygial Fibroblast Proliferation.

Lee, Jong Soo; Jung, Do Young; Kim, Yoo Seon

Original Article

Journal of the Korean Ophthalmological Society 1998;39(7):1410-1416.
Published online July 1, 1998.

The Effect of Mitomycine C (MMC) on Inhibition of Pterygial Fibroblast Proliferation.

Jong Soo Lee, Do Young Jung, Yoo Seon Kim

¹Department of Ophthalmology, College of Medicine, Pusan National University, Pusan, Korea.
²Central Research Laboratory, Pusan National University Hospital,Pusan, Korea.

익상편 섬유모세포의 증식억제에 관한 Mitomycin C 의 효과

이종수(Jong Soo Lee),정도영(Do Young Jung),김유선(Yoo Seon Kim)

Abstract

The purpose of this study is to investigate that the biological effect of mitomycin C(MMC) in cellular metabolic activity and morphological change on the ptreygium fibroblast in vitro by MMC concentration and duration of exposure used clinically. Human pterygial fibroblasts were exposed for threeminute and five-minute to MMC 0.002%, 0.004%, 0.01%, 0.02%, 0.04%, and DMEM(control). MTT based colorimetric assay was performed to assess the metabilic activity, inhibition of fibroblast proliferation on the MMC concentration and exposure time. The higher the concentration of MMC, and longer the duration of exposure time, the absorbance of spectrometer are decreased. The metabolic activity of fibroblasts were inhibited by 50% at least only over MMC 0.02% for five-minute expoure time. In histological findings, the higher the concentration and longer the duration of MMC exposure time, the enlargement of many mitochondira and rough endoplasmic reticulum without nuclear damage were more distinctly appeared. Especially, the ptergial fibroblast has more severe cytoplasmic damage at a five-minute exposure to MMC 0.02% than a three-minute exposure to MMC 0.04%. For inhibition of fibroblast proliferation, in case of using MMC should be at least over 0.02% concentration for five-minute exposure time. Arthors think that the experimental and clinical research on the duration of MMC exposure time as well as the concentration MMC, should be need to evaluate the effect on inhibition of cellular proliferation.

Key Words: Mitomycin C;MTT based colorimetric assay;Pterygial fibroblast;Proliferation