| Aqueous and Vitreous Concentration of Mitomycin C Administered Topically after Holmium Laser Sclerostomy in Rabbits. |
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Jae Hun Hyun, Jong Hyuck Lee, Soon Hyun Kim |
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Department of Ophthalmology, Wonju College of Medicine, Yonsei, University, Korea. |
| 토끼에서 홀미움레이저 공막절개술 후 국소점안 mitomycin C의 전방 및 유리체내의 농도 |
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현재헌(J H Hyun),이종혁(J H Lee),김순현(S H Kim) |
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| Abstract |
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Mitomycin C is an antibiotic isolated from the broth of Streptomyces caespitosus which has been used as the adjunctive drug to reduce the fibroblast proliferation. Holmium laser sclerostomy can minimize conjunctival damage. We examined pharmacokinetics of mitomycin C following topical administration. Holmium laser sclerostomy was performed on one eye of each 15 rabbits and the other eye was served as a control. Next day, mitomycin C solution(0.4mg/ml) was administered by topical drops in both eyes of each rabbit. The concentrations of mitomycin C in the aqueous and the vitreous compartments of operated and control eyes were determined by HPLC at 0.5, 1, 2, 3, 6 hours after topical administration. The peak aqueous concentration was 0.07 microgram/ml in operated eyes and 0.06 microgram/ml in control eyes. The peak vitreous concentration was 0.10 microgram/ml in operated eyes and 0.09 microgram/ml in control eyes. The levels of detected mitomycin C at all time intervals were higher than the IC50(0.002 microgram/ml) for the inhibition of conjunctival fibroblast proliferation. These results suggest that topical mitomycin C easily crosses the intact and minimally damaged conjunctiva and therefore increases the success rate of Holmium laser sclerostomy. |
| Key Words:
Holmium laser sclerostomy;Mitomycin C;Topical administration |
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