Interaction of Attachment Complex and Anchoring Fibrils following Corneal Culture.

Ko, Myung Kyoo

Original Article

Journal of the Korean Ophthalmological Society 1996;37(5):854-861.
Published online May 1, 1996.

Interaction of Attachment Complex and Anchoring Fibrils following Corneal Culture.

Myung Kyoo Ko

Department of Ophthalmology, College of Medicine, Hanyang University, Seoul, Korea.

각막조직 배양후 부착복합체와 Anchoring Fibrils 과의 관계

고명규(Myung Kyoo Ko)

Abstract

This study was performed to elucidate the structural changes of the attachment complex in the cultured cornea. The three normal corneal tissues were used in this study. The ultrastructural changes of the attachment complex were observed by electron microscopy in one corneal tissue which was not cultured and cultured for 6 days and two corneal tissues which were cultured 10 days and 20 days, respectively. The cornea cultured for 6 days showed changes in the electron density of the hemidesmosome. The basal lamina was focally detached from the cytoplasmic wall of the basal epithelial cells in the cornea cultured for 10 days. The anchoring fibrils within the nuded corneal stroma, which was cultured for 20 days, were markedly decreased in number. These findings suggest that the normal basal epithelial cell, hemidesmosomes and basal lamina might be the essential factors to maintain the networks of normal anchoring fibrils in corneal stroma.

Key Words: Anchoring fibril;Attachment complex;Basal lamina;Cornea