Antiangiogenic Effects of Retinoids on Corneal Neovascularization.

Choi, Yong Suk; Yu, Young Suk; Lee, Jag Jun; Chung, Hum; Lee, Jaeheung; Chang, Hong Leen

Original Article

Journal of the Korean Ophthalmological Society 1996;37(9):1413-1421.
Published online September 1, 1996.

Antiangiogenic Effects of Retinoids on Corneal Neovascularization.

Yong Suk Choi, Young Suk Yu, Jag Jun Lee, Hum Chung, Jaeheung Lee, Hong Leen Chang

Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.

각막신생혈관에 대한 retinoids의 항혈관형성효과

최용석(Yong Suk Choi),유영석(Young Suk Yu),이재준(Jae Jun Lee),정흠(Hum Chung),이재흥(Jae Heung Lee),장봉린(Bong Leen Chang)

Abstract

We investigated the effect of topical application of retimids on the corneal neovascularization in the rat induced by chemical cauterization. The center of corneas of Wistar rats were Cauterized with a silver/potassium nitrate applicator for 5 seconds. And then they were treated topically with 0.1%, 0.2%, 0.5%, 2.0% all-trans retimids (retinol, retinoic acid, retinaldehyde) and dimethyl sulfoxide as a control four times a day. After appiications of eye drops for 5 days, each rat was kined and then perfused with a mixture of 11% gelatin 10% India ink-lactated Ringer's solution. Corneal flat preparation were made, and then the percent of the corneal area occupied by blood vessels were analized by computerized image analyzer. Percent vascularization of 0.1% and 0.2% retnoids were not significantly different from control group(p>0.05). Percent vascu1arization of 0.5% retinaldehyde and all 2.0% retnoids were significantly lower than the contml group(p>0.5). These naturally occurring retinoids were effective in antiarlgiogenesis of rat cornea at relatively high concentrations (>0.5%), when treated topicany, It will be necessary to further study on more potent antiangiogenic, at lower concentration, synthetic retimids for the treatment of angiogenic diseases.

Key Words: Antiangiogenic effect;Chemical cauterization;Computerized image analyzer;Corneal neovascularization;Retinoids