Journal of the Korean Ophthalmological Society 1997;38(1):86-93.
Published online January 1, 1997.
Comparison of the Retinal Microvasculature between Normal and Diabetic Rats Using Trypsin & DNase Digestion Technique.
Ji Han Ryou, Hae Jung Paik, Mee Gyeong Park
Department of Ophthalmology, National Police Hospital, Seoul Korea.
Trypsin 과 DNase 를 이용한 당뇨쥐의 망멱혈관 구성세포변화에 대한 비교연구
류지한(Ji Han Ryou),백혜정(Hae Jung Paik),백미경(Mee Gyeong Park)
Abstract
Trypsin and DNase digestion technique has become a retinal digestion technique for studying diabetic retinopathy. We tried osmotic digestion method with DNase and compared the quality of preparation of retina and microvascular change with trypsin digestion in normal and diabetic rats. Streptozotocin-induced diabetic rats were sacrificed at 9, 18, 27weeks in 6 rats. Right retinas were digested with 3% trypsin while left wer digested with 0.1% DNase. For control, 18 normal rats were sacrificed at the same time. DNase was superior to trypsin for retinal preparation on the stainability, the degree of separating nonvascular fissue from vascular net, the degree of preservation of vascular net in normal & diabetic rats. In diabetic rats, the number of pericyte decreased significantly with age, which not in normal rats(p=0.0023) We suggest DNase digestion technique as an new alternative for trypsin digestion technique in the study of microvascular change of diabetic retinopathy.
Key Words: Diabetes mellitus;DNase;Endothelial cell;pericyte;Trypsin


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