| Effects of synthetic Inhibitor of Metalloproteinase and Cyclosporin A on Corneal Haze and Collagen Synthesis after Excimer Laser Photorefractive Keratectomy in Rabbits. |
|
Jin Ho Chang, Won Ryang Wee, Hum Chung, Jin Hak Lee |
1Department of Ophthalmology, Seoul City Boramae Hospital.
2Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. |
| 가토에서 굴절교정레이저각막절제술후 발생한 각막 혼탁 및 교원질 생성에 대한 합성 금속단백분해효소 억제제 및 사이클로스포린A의 효과 |
|
장진호(Jin Ho Chang),위원량(Won Ryang Wee),정흠(Hum Chung),이진학(Jin Hak Lee) |
|
|
|
|
| Abstract |
|
Photorefractive keratectomy(PRK) was performed on 60 rabbit eyes, and synthetic inhibitor of metalloproteinase(SIMP) and cyclosporin A(CsA) was topically administered and their effects on coreneal haze were evaluated. They were randeomized to one of four groups: group A received topical SIMP, group B received topical CsA, group C received both SIMP and CsA, and group D received vehicles. At one, two, four, and six weeks after surgery, slit lamp examination was performed, and haze gradings were recorded. Light microscopy, together with immunohistochemistry using antibodies against collagen types III, IV, and VI were performed in corneas from all groups. Slit lamp examination and light microscopy revealed that SIMP significantly reduced corneal haze after PRK and subepithelial deposition of newly synthesized extracellular matrix, but CsA did not. By immunohistochemistry, deposition of types III and IV collagen was noted in ablated area of all groups. Positive staining for type III collagen was less frequent in groups treated with SIMP than in groups not treated with SIMP. In conclusion, SIMP significantly reduced the synthesis of type III collagen in treated area as well as corneal haze after excimer laser PRK in rabbits. |
| Key Words:
Corneal haze;Excimer lasr;Immunohistochemistry;Matrix metalloproteinase;Photorefractive keratectomy;Synthetic inhibitor of metalloproteinase |
|
PDF Links
Download Citation
