The Effect of Anticancerous Drug in the Fiboblast-Mediated Collagen Matrix Contraction.

Kwak, Hyung Woo; Park, Chang Soo; Jin, Kyung Hyun; Kim, Jae Myung

Original Article

Journal of the Korean Ophthalmological Society 1997;38(5):805-816.
Published online May 1, 1997.

The Effect of Anticancerous Drug in the Fiboblast-Mediated Collagen Matrix Contraction.

Hyung Woo Kwak, Chang Soo Park, Kyung Hyun Jin, Jae Myung Kim

¹Department of Ophthalmology, College of Medicine, Kyung Hee University.
²Department of Ophthalmology, Kang Nam Hospital.

인공증식막 형성 억제에 관한 연구

곽형우(Hyung Woo Kwak),박창수(Chang Soo Park),진경현(Kyung Hyun Jin),김재명(Jae Myung Kim)

Abstract

In vitro PVR(Proliferative vitreoretinopathy) models allow identification of factor which may inhibit porliferation and contraction. In this study we evaluated the contraction of collagen matrix by choroidal fibroblast and the inhibition of contraction by antipoliferative drug. Each antiproliferative drug showed inhibition of collagen matrix contraction : Colchicine (0.1microgram/ml), Cytochalacin (0.05microgram/ml), Puromycin(10microgram/ml). Transmission electron microgram of collagen matrices containing colchicine, cytochalacin or puromycin showed no collagen fiber surrounding choroid fibroblast and showed cell destruction. Scanning electron microgram of collagen matrices containing colchicine, cytochalacin and puromycin showed that collagen fibers were well preserved without distortion. Colchicine, cytochalacin and puromycin are effective inhibitor of cell mediated contraction in additon to it`s potent antiproliferative effect wherease Interfereon has no anticontractile effect. The current study present a model to investigate the effect of antiproliferative drug on fibroblast mediated collagen matrix contraction.

Key Words: Proliferative vitreoretinopathy;colchicine;cytochalacin;puromycin;Interferon;collagen;fibroblast