| Comparison between CT-HRP and Bartha Strain of Pseudorabies Virus as a Neurotracer in the Rat Visual System. |
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Ji Myong Yoo, Han Gyu Kim, Bong Hee Lee, Sang Do Bae, Jong Jung Kim, Ji Hong Bae, Jong Moon Park, Joon Kyeong Song |
1Department of Ophthalmology, College of Medicine, Gyeongsang National University, Korea.
2Department of Anatomy, College of Medicine, Gyeongsang National University, Korea. |
| 흰쥐 시로 추적연구에 있어 신경추적자로서 CT - HRP 와 Pseudorabies 바이러스의 비교 |
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유지명(Ji Myong Yoo),송준경(Jun Kyeong Song),김한규(Han Gyu Kim),이봉희(Bong Hee Lee),배상도(Sang Do Bae),김정중(Jong Jung Kim),배지홍(Ji Hong Bae),박종문(Jong Moon Park) |
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| Abstract |
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To investigate the applicability of the Bartha strain of pseudorabies virus(PRV-Ba) as a neurotracer in the visual system, we performed immunohistochemical study of the rat brain after intravitreal injection of the PRV-Ba. Cholera toxin-horseradish peroxidase(CT-HRP), a well known neurotracer, was also injected into the vitreous cavity for comparison. After given survival time the brain was removed and processed immunohistochemical stain for PRV-Ba and neurohistochemical stain for CT-HRP. The CT-HRP positive reactions were observed almost contralaterally in optic chiasm, optic tract, suprachiasmatic nucleus(SCN), olivary pretectal nucleus(OPT), all three subdivision of lateral geniculate body, superficial gray layer and optic layer of superior colliculus and medial, lateral, dorsal terminal nucleus of accessory optic tract. PRV-Ba immunopositive reaction was observed in SCN and OPT, but not in nerve fiber system and dorsal lateral geniculate body. Also PRV-Ba positive cells were distributed mainly in intermediate layer and weakly distributed in superficial layer of the superior colliculus. This study suggests that PRV-Ba is not a useful neurotracer in studying the visual system of the rat. |
| Key Words:
CT-HRP;Neurotracer;PRV-Ba;Visual system |
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