Distribution of Glycoconjugates During Wound Healing After Argon Laser Photocoagulation in Pigmented Rabbit Retina. |
Hee Seong Yoon, Sae Heun Rho, Boo Sup Oum, Young Hyun Yoo |
1Department of Ophthalmology, College of Medicine, Dong-A University, Korea. 2Department of Ophthalmology, College of Medicine, Busan National University, Korea. 3Department of Anatomy, College of Medicine, Dong-A University, Korea. |
유색가토의 망막에 아르곤레이저 광응고시 상처치유에 있어 복합당질의 분포에 관한 연구 |
윤희성(Hee Seong Yoon),노세현(Sae Heun Rho),엄부섭(Boo Sup Oum),유영현(Young Hyun Yoo) |
|
|
Abstract |
Lectin is a non-immunological glycoprotein and binds specifically to carbohydrate terminals in tissue. Lectin histochemistry using 10 different biotinylated lectins was performed to investigate the effects on thirty-two pigmented rabbit retinas during wound healing. The results are as follows: 1) In normal retina. a) WGA, RCA I, and LCA were bound to the internal limiting membrane. b) WGA, RCA I, LCA, and PNA were bound to the photoreceptor layer. c) WGA, RCA I, LCA, SJA, ConA and BSL I were bound to the basal side of retinal pigment epithelium. d) PNA was bound to cone cell only. e) SBA, DBA, and UEA I didn't bind to any layers of retina. 2) In photocoagulated wound. One day after photocoagulation WGA, LCA and RCA I began to show increased reaction. At 3 and 5 days these lectins sustained reactivity. At 7 days increased reactivity began to decrease or disappear from wound. Macrophages had positive reaction to BSL I, WGA, LCA, and RCA I. In conclusion these results indicate that a-mannose, a glucose, a, beta-galactose, N-acetylglucosamine, and N-acetylneuramic acid are present in glycoconjugates of normal rabbit neural retina. It seems that some glycoconjugates may be related to vitreoretinal, retinal and chorioretinal adhesion in normnal retinal, and after argon laser photocoagulation. WGA-binding, LCA-binding and RCA I-binding glycoconjugates may play a part in cell adhesion during early wound healing. |
Key Words:
Argon laser photocoagulation;Glycoconjugate;Wound healing |
|