The Effect of a Collagen Shield on Concentrations of Tobramycin in Corneas.

Baik, Jong Min; Kim, Ki San; Oh, Joon Sup

Original Article

Journal of the Korean Ophthalmological Society 1990;31(2):155-159.
Published online February 1, 1990.

The Effect of a Collagen Shield on Concentrations of Tobramycin in Corneas.

Jong Min Baik, Ki San Kim, Joon Sup Oh

Department of Ophthalmology, College of Medicine, Keimyung University, Daegu, Korea.

Tobramycin 의 각막내 침투에 미치는 Collagen Shield 의 효과에 관한 연구

백종민(Jong Min Baik),김기산(Ki San Kim),오준섭(Joon Sup Oh)

Abstract

Collagen shields made of porcine scleral collagen were immersed in a solution containing tobramycin sulfate(40 mg/ml) for five minutes, then applied to rabbit corneas. One, four and eight hours after application, the corneas were assayed for antibiotics by agar diffusion bioassay. We divided study groups as 4 groups, that is, collagen shield, subconjunctival, therapeutic soft contact lens and topical group, and compared the corneal concentradon of tobramycin in each group. Shields immersed in 40 mg/ml tobramycin produced significantly higher concentrations of tobramycin in the cornea at one and four hours than those of the other groups. Tobramycin concentrations in cornea eight hours after application became lower than those of one and four hours after application but, higher than minimal inhibitory concentration of Pseudomonas aeruginosa. Collagen shield containing antibiotics can serve as a vehicle for drug delivery and may prove superior to current methods for the initial treatment of bacterial keratitis, and preoperative and postoperative antibiotic prophylaxis.

Key Words: agar diffusion bioassay;collagen shield;corneal concentration;tobramycin