The Effect of Saponin on the Na+ - K+ Activated ATPase of Cattle Lens Epithelial Cell. |
We Gon Chang, Kwang Lim Koo, Byung Nam Kang |
1Department of Ophthalmology Seoul Red Cross Hospital, Korea. 2Kang's eye Clinic, Korea. |
Saponin 이 소의 수정체 상피막의 Na+ - K+ ATPase 의 활성도에 미치는 영향 |
장외곤(We Gon Chang),구광림(Kwang Lim Koo),강병남(Byung Nam Kang) |
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Abstract |
The effect of saponin on the Na+ - K+ ATPase of anterior capsule and epithelium of cattle lens has been investigated. The experiment also designed to determine the cation of saponin on the Na+ - K+ ATPase activity in anterior capsule and epithelium of cattle lens. The following results are observed. 1. The Na+ - K+ ATPase activity of cattle lens epithelial cell membrane is inhibited by low concentration of saponin, but increased by high concentration. The strongest activity showed at the dose of 50mg% saponin concentration. 2. The activating effect of saponin on the cattle epithelial cell membrane Na+ - K+ ATPase activity is inhibited by ouabain, but the stimulation of the Mg++ ATPase by high concentration of saponin is not inhibited by ouabain. 3. The activity ratio of Na+ - K+ ATPase by high concentration of saponin is increased by raising the sodium concentration but decreased by raising the potassium concentration. 4. The Na+ - K+ ATPase activity is increased by small amount of calcium but completely inhibited by over 5mM concentration of calcium. |
Key Words:
Cataract;Inorganic phosphate;Lens epithelium Na+ - K+ ATPase;Saponin |