Effect of Conditioned Medium of Cultured Retinal Pigment Epithelium on Proliferation of Vascular Endothelial Cells in Bovine; In Vitro Study.

Kim, Jae Ho; Lee, Young Chun; Hahn, Tae Won; Huh, Warne

Original Article

Journal of the Korean Ophthalmological Society 1992;33(4):381-387.
Published online April 1, 1992.

Effect of Conditioned Medium of Cultured Retinal Pigment Epithelium on Proliferation of Vascular Endothelial Cells in Bovine; In Vitro Study.

Jae Ho Kim, Young Chun Lee, Tae Won Hahn, Warne Huh

Department of Ophthalmology, Catholic University Medical College Kangnam St. Mary's Hospital, Seoul, Korea.

배양된 소 (牛) 망막색소상피세포의 조절된 배양액이 혈관내피세포의 증식에 미치는 효과 , In Virto 연구

한태원(Tae Won Hahn),김재호(Jae Ho Kim),이영춘(Young Chun Lee),허원(Warne Huh)

Abstract

Neovascularization is a serious complication and major cause of visual loss in ocular diseases such as diabetic retinopathy and proliferative vitreoretionpathy. So there have been many attempts to inhibit neovascularization. Most inhibitors of neovascularzation so far identified have been extracted from lens and vitreous, and recently retinal pigment epitheliumin in culture also secrete angiogenic inhibitors. We cultured retinal pigment epithelial cell, vascular endothelial cell of Umblical vein and subconjunctival fibroblast of bovine. We studied the inhibitory effect in each of retinal pigment epithelium conditioned medium (RPE-CM) and fibroblast-CM on proliferation and migration of vascular endothelial cell comparing the effect of normal media group. In RPE-CM, proliferation and migration of vascular endotrelial cells were inhibited and the inhibition was dose-dependant. But in fibroblast-CM, we didn't observe the inhibitory effect These results suggest that in RPE-CM, there were certam substances to inhibit vascular endothelial cell proliferation and migration. Further research should be continued to detect that substance in molecular biological level.

Key Words: Neovascularization;RPE cell;RPE-CM;Fibroblast-CM proliferation;Migration