Immunohistochemical Study of the Endotoxin Induced Uveitis in Rat.

Park, Jong Moon; Yoo, Ji Myong; Song, Joon Kyeong

Original Article

Journal of the Korean Ophthalmological Society 1992;33(6):583-588.
Published online June 1, 1992.

Immunohistochemical Study of the Endotoxin Induced Uveitis in Rat.

Jong Moon Park, Ji Myong Yoo, Joon Kyeong Song

Department of Ophthalmology, College of Medicine, Gyeong Sang National University, Korea.

세균내독소에 의하여 유발된 흰쥐 포도막염에서의 림파구의 변화

박종문(Jong Moon Park),유지명(Ji Myong Yoo),송준경(Joon Kyeong Song)

Abstract

Using immunohistochemical technique, we observed time-sequentially to T and B lymphocyte in the ciliary body of the endotoxin induced uveitis in rat. The primary antibodies for immunohistochemical stain of the lymphocyte were IgM heavy chain, IgG heavy chain, T suppressor/cytotoxic (CD8) and T helper/macrophage (CD4) was performed in the frozen section. The intensity of immunohistochemical reaction for surface IgM positive cells increased from 12hours and reached most strong intensities at 24hours after endotoxin injection and then decreased rapidly, but the intensity for surface IgG positive cells increased from 48hours and sustained 96hours. There were no significant changes of intensity of immunohistochemical reaction for T cells except slightly increased T suppressor/cytotoxic cells (CD8).

Key Words: Endotoxin;Immunohistochemical technique;T and B lymphocyte