Journal of the Korean Ophthalmological Society 1992;33(7):678-683.
Published online July 1, 1992.
Culture of Retinal Pigment Epithelial Cells on Collagen Membrane.
Sung Chul Lee
Department of Ophthalmology, Yonsei University, College of Medicine, Seoul, Korea.
미세공이 있는 반투과성막을 이용한 망막색소상피세포의 배양
이성철(Sung Chul Lee)
Abstract
Cell culture technique has been used for the study of retinal pigment epithelial cells. Retinal pigment epithelial cells from the pigmented rabbits were cultured on the plastic culture plates and on the semipermeable collagen membranes separately. The appearance of the cultured cells and the characteristics of cell proliferation was observed. Also, the presence of the fluorescent material excreted from the cultured cells was investigated Mitotic activity of the retinal pigment epithelial cells initiated 3 days after inoculation The cell density appeared to influence more or less the rate of proliferation which was evident by the rapid cell growth in the area of high cell density. Compared to the retinal pigment epithelial cells on the plastic plates, the cells on the collagen membranes showed slower rate of growth and were transformed into the spindle shaped cells. So the collagen membranes appeared to interfere with cell spreading. No fluorescence was detected from the material excreted through the collagen membranes.
Key Words: Cell Culture;Retinal pigment epithelium;Collagen


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