The Distribution of Fibronectin in the Experimental Keratitis of Rabbit.

Lee, Doo Suck; Kim, Jae Duck

Original Article

Journal of the Korean Ophthalmological Society 1987;28(2):319-326.
Published online February 1, 1987.

The Distribution of Fibronectin in the Experimental Keratitis of Rabbit.

Doo Suck Lee, Jae Duck Kim

Department of Ophthalmology, College of Medicine, Won-Kwang University, Korea.

가토의 실험적 각막염에 있어서 Fibronectin 분포에 관한 면역세포화학적 검색

이두석(Doo Suck Lee),김재덕(Jae Duck Kim)

Abstract

The distribution of fibronection was investigated in the carrageenan-induced keratitis of rabbitis. Keratitis was induced by the injection of carrageenan into corneas of the rabbits, and specimens were taken at various intervals. Histological examinations were done with H and E, silver impregnation and PTAH stains. The distribution of fibronectin was observed by using avidin-biotin-peroxydase(ABC) technique. The results were follow. 1. The histological changes in early stages showed acute inflammatory exudate in stroma. After two weeks, granulation tissues were formed. And then after the fourth week, the granulation tissues began to mature with the apperance of fascicle of collagen fibers and thickened epithelium. 2. Fibronectin was demonstrated in a fibrillar appearance in the acute inflammatory exudate of the stroma, which was normally absent. With the development of chronic inflammatory lesions and granulation tissues, the amount of fibronectin was increased, relative to the increase and codistribution of reticular fibers, but with the appearance of mature collagen fibers, the amount was markedly reduced. 3. The fibronectin which was absent in the normal epithelium was prominent in the regenerating epithelium.