Glycosylation of Human Lens Capsule(Basement Membrane) Protein in Diabetes Mellitus.

Baek, Nam Ho

Original Article

Journal of the Korean Ophthalmological Society 1987;28(2):327-332.
Published online February 1, 1987.

Glycosylation of Human Lens Capsule(Basement Membrane) Protein in Diabetes Mellitus.

Nam Ho Baek

Department of Ophthalmology, St. Mary's Hospital, Catholic Medical College, Seoul, Korea.

당뇨병에서 수정체낭 단백의 Glycosylation 치

백남호(Nam Ho Baek)

Abstract

Human lens capsules(basement membrane of lens epithelium) were isolated from surgically removed cataractous lenses of diabetic and nondiabetic patients with senile cataracts. Basement membranes were purified from lens capsules, using the detergent Triton X-100 and the extent of glycosylation was determined with a colorimetric reaction that detected carbohydrates in ketoamine linkage with proteins. Furthermore, the level of glycosylation of lens capsule basement membrane from diabetics was significantly higher than that in their nondiabetic counterparts and capsule basement membranes were confirmed to be glycosylated.