J Korean Ophthalmol Soc > Volume 58(12); 2017 > Article
Journal of the Korean Ophthalmological Society 2017;58(12):1333-1340.
DOI: https://doi.org/10.3341/jkos.2017.58.12.1333    Published online December 15, 2017.
Effects of Human Serum on Human Corneal Epithelial Cells in Vitro.
Young Min Park, Jae Sung Park, In Ho Lee, Jong Soo Lee
1Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea.
2Department of Ophthalmology, Gyeongsang National University Changwon Hospital, Changwon, Korea.
3Danggam Choi Eye Clinic, Busan, Korea.
4Department of Ophthalmology, Pusan National University School of Medicine, Yangsan, Korea. jongsool@pusan.ac.kr
인체 혈청이 배양된 인체 각막상피세포에 미치는 영향
경상대학교 의학전문대학원 안과학교실1, 창원경상대학교병원 안과2, 당감최안과의원3, 부산대학교 의학전문대학원 안과학교실4
Correspondence:  Jong Soo Lee,
Email: jongsool@pusan.ac.kr
Received: 16 March 2017   • Revised: 27 June 2017   • Accepted: 29 November 2017
To investigate the effect of human serum on corneal epithelial cells. METHODS: Changes of corneal epithelial cells were evaluated after 1, 4, 12, and 24 hours (hrs) of exposure to various concentrations of human serum (3, 5, 8, and 16%). Cellular metabolic activity and the extent of cellular damage were measured. Effect of human serum on cell migration was also examined. Concentration of procollagen type-I COOH-terminal peptide (PIP), epidermal growth factor (EGF), and laminin after exposure to human serum was further observed. RESULTS: In every concentration of human serum, metabolic activity of the corneal epithelial cells temporarily decreased at 4 hrs of exposure and recovered to baseline levels afterward. With the same exposure time, there was no statistically significant difference in metabolic activity between the human serum-exposed group and the control group. Cellular toxicity of human serum exhibited a time- and dose-dependent relationship. Cellular migration was observed after 24 hrs of exposure to 5% concentration of human serum and after 12 hrs of exposure to 8% and 16% concentration of human serum. The PIP, EGF, and laminin titers increased in time- and dose-dependent manners. CONCLUSIONS: Human serum does not decrease the metabolic activity of corneal epithelial cells as the concentration and exposure time increase, but it can induce cytotoxicity. Considering cellular migration, a serum concentration of 5% or higher should be used.
Key Words: Corneal epithelium;Metabolic activity;Scratch wound assay;Serum;Toxicity

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